By Alan A. Boulton, Glen B. Baker
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Extra resources for Amines & Their Metabolites (NEUROMETHODS) (Neuromethods 2 Series I Neurochemistry)
Anton A. H and Sayre D. F. (1968) Possible sources of error m solvent extraction procedures for catecholammes Eur J Pharmacol 4, 435440. 32 Baker, Butterworth, and Dewhurst Anton A H and Sayre D F. (1969) A modified fluorometric procedure for tissue histamme and its distribution m various animals. I Pharmacol. Exp Ther. 166, 285-292 Ashcroft G. W. and Sharman D F (1962) Drug-induced changes m the concentration of 5-OR mdolyl compounds m cerebrospmal fluid and caudate nucleus Brat ] Pharmacol. 19, 153-160.
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As little as 10-‘2-10-‘4 mol could be detected, but the method could not be used for quantitation. In his detailed 1974 review, Osborne (1974) carefully outlined microprocedures involving reaction with dansyl-Cl to measure the amines 5-HT, T, NA, A, OA, GABA, and other amino acids. Such derivatives are highly fluorescent and allow detection of as little as 5 pm01 on thin-layer chromatograms by direct fluorimetry. If radiolabeled dansyl-Cl is used and measured by autoradiography, as little as 1 pmol may be detected.
Amines & Their Metabolites (NEUROMETHODS) (Neuromethods 2 Series I Neurochemistry) by Alan A. Boulton, Glen B. Baker