Download PDF by Halkin, Francois: Bibliotheca Hagiographica Graeca (BHG), Vol. II: Ioannes

By Halkin, Francois

«Bibliotheca hagiographica graeca « представляет собой каталог греческих агиографических материалов, в том числе древних литературных произведений о жизни святых, расположенных в алфавитном порядке.

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Obtained with the Affymetrix E. coli GeneChip™ when these messageenriched RNA samples are used to generate biotin-labeled RNA targets (see Chapter 7). Typically, this procedure gets rid of about 90 percent of the ribosomal RNA in a bacterial total RNA preparation. Other protocols for the preparation and hybridization of prokaryotic and eukaryotic labeled targets to Affymetrix GeneChips™ as well as nylon filters and glass slide arrays also are described in Appendix A. 42 Gene expression profiling experiments Problems associated with target preparation with polyadenylated mRNA from eukaryotic cells To date, all reported eukaryotic DNA array experiments have been performed with targets prepared from poly(A)ϩ mRNA isolated with oligo(dT) chromatography columns or by synthesizing cDNA targets with oligo(dT) primers.

At this resolution, even the highest density arrays can be accurately 20 DNA array readout methods scanned. Scanning at this resolution, however, requires several minutes. For this reason, manufacturers of confocal laser scanners provide the option to scan at lower resolutions ranging down to 50 ␮m. This is useful for rapid pre-scanning of arrays for sensitivity and scanning alignment adjustments. There are three ways to scan an array – either move the array or move the laser, or both. In general systems that move the array under a fixed laser source are preferred because of their increased light-gathering efficiency.

Coli mRNA are rapid and message specific, the “clearance” rate for message degradation intermediates to free nucleotides must occur at a more constant rate. Thus, the relative mRNA expression levels measured with the random hexamer labeled probes should be more closely related to their rates of synthesis and, therefore, their relative abundance in the cell. This conclusion is supported by two observations. First, nearly equal levels of expression of genes of a common operon are observed with the random-hexamer-generated targets, but not with the targets generated with the 3Ј message-specific primers.

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Bibliotheca Hagiographica Graeca (BHG), Vol. II: Ioannes Calybita-Zoticus by Halkin, Francois


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