Read e-book online Blood Filtration and Blood Cell Deformability: Summary of PDF

By J. A. Dormandy (auth.), John Dormandy M.D., F.R.C.S. (eds.)

ISBN-10: 0898387140

ISBN-13: 9780898387148

ISBN-10: 940095008X

ISBN-13: 9789400950085

H. J. Meiselman From the theoretical reviews of Dr. Skalak, it truly is transparent that white cells can considerably effect the pressure-time profile of a pink cell/white telephone suspen­ sion, and that the presence of even a small quantity of quite inflexible white cells may have a profound impact at the filtration strain throughout the latter component of a filtration scan. Conversely, white cellphone results, despite their relative stress, are proven to have simply minimum results through the very early (i. e. , 0-2 seconds) stages of the filtration method. Dr. Chien's experimental information help those theoretical reports, in that white cells of other mechan­ ical houses express assorted pressure-time curves; pressure-time info for combinations of leucocytes convey shapes which might be estimated from the habit of fairly homogeneous mobile populations. The insensitivity of the very early parts of the filtration method to white cells is back mirrored within the calculations made via Dr. Hanss. utilizing the nominal dilutions, white telephone concentrations and the entire quantity of filtered mobilephone suspension, he shows that typically under 1 pore out of a hundred is susceptible to blockage via white cells. He therefore concludes that, on the 1% accuracy point, preliminary filtration facts shouldn't be suffering from mechanical pore blockage by means of white cells. Experimental experiences via Dr. Lowe and Dr. Stuart query the WBC­ insensitivity of the early element of the filtration strategy. utilizing a continuing move method, Dr.

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04) 35 stored at room temperature for 2 h prior to measurement of red cell rheology using the above methods. References 1. A. L. (1976). A simple method for measuring erythrocyte deformability. Journal of Clinical Pathology, 29, 855-858. 2. G. and La Puma, I. (1978). Reduced erythrocyte deformability in diabetes. Diabetes, 27, 895-901. 3. A. (1970). Automatic recording of the rate of packing of erythrocytes in blood by a centrifuge. Physics in Medicine and Biology, 15,9-14. 4. Dintenfass, L. (1976).

USA). Volume-time data for RBC in various Tris buffers are shown in Fig. 0 indicates no change from the initial control condition. Note that Tris causes an increase in cell volume which is a function of time, concentration, and temperature. Since the relative accuracy of the volume measuring system is in the order of1 %, significant volume increases occurred at 21° C for 5 mM at 38 3 hours, for 10 mM at 2 or more hours, and for 20 mM and greater at 1 hour and beyond. At 37° C, significant increases were noted for 5 mM at 2 hours and beyond, for 10 mM at 1 hour and beyond, and for 20,40 and 50 mM at one-half hour and beyond.

Thus, based solely on volume stability, HEPES appears to be the buffer of choice; additional studies of its effects, if any, on RBC mechanical properties will be needed prior to acceptance of this agent as the ideal 'non-plasma' suspending medium. 0 References 1. D. l. (1975). Effects of Tris and histidine on human erythrocytes and conditions influencing their mode of action. Biochimica et Biophysica Acta, 382, 634-649. 2. Hanss, M. (1983). Plasma and suspending medium. In: Red Cell Deformability and Filterability (Ed.

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Blood Filtration and Blood Cell Deformability: Summary of the proceedings of the third workshop held in London, 6 and 7 October 1983, under the auspices of the Royal Society of the Medicine and the Groupe de Travail sur la Filtration Erythrocitaire by J. A. Dormandy (auth.), John Dormandy M.D., F.R.C.S. (eds.)


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